The owner of the female python, Neth Vy says he brought the snake home 16 years ago and it has brought them prosperity.
Neth was told by a fortune teller that not wedding his snake to another would bring bad luck.
The two-hour ceremony was held in Svay Rolum village and after two Buddhist monks blessed the pythons the villagers showered them with flowers.
The male python, Kroung Pich was brought home by a childless woman who regards it as her son. Animism is the belief that spirits inhabit living things and is prevalent in Cambodia.